A method for the quantitative estimation of bacteria from mangrove sediments     

A.H. Dye 《Estuarine, Coastal and Shelf Science》1983,17(2):207-212

A simple and rapid method for the quantitative extraction of bacteria from high silt/clay-content muds associated with mangrove swamps is described. Mud samples are homogenized in filtered sea water and the supernatant is diluted and stained with Acridine Organge. The stained material is trapped on a 0·45 μm membrane filter and bacteria are counted under a fluorescence microscope. Samples may be pre-fixed in 5% formalin if they cannot be examined immediately.  相似文献   

The relationship between abundances of meiofauna and their suspected microbial food (diatoms and bacteria)     

Paul A. Montagna  Bruce C. Coull  Teresa L. Herring  Bettye W. Dudley 《Estuarine, Coastal and Shelf Science》1983,17(4):381-394

Samples were taken bi-weekly for one year at a sand site and a mud site in the North Inlet Estuary, Georgetown, South Carolina, for meiofauna, their suspected microbial food (bacteria and diatoms), and associated physical factors. Linear regression techniques were used to correlate food abundance and physical factors with the density of meiofaunal taxa. At both sites diatoms positively correlated with meiofauna taxa, but bacteria did not. Physical factors were not correlated with meiofaunal or microbial abundances at the sand site. Whereas, at the mud site meiofauna and diatom abundances were positively correlated with the depth of the redox layer and inversely correlated with temperature. Peaks of meiofaunal abundance did not follow peaks of food abundance. Analysis of copepods at the species level indicated that taxa response was due to the response of the dominant species. Even though some correlations existed, this study suggests that copepod species and meiofauna at the gross taxonomic level do not respond to changes in potential food abundance. Physical factors apparently influence both meiofauna and diatoms in the same fashion. However, bacterial abundance was not positively correlated with any of the factors studied.  相似文献   

Cultivable bacteria from bulk water,aggregates, and surface sediments of a tidal flat ecosystem     

Heike Stevens  Meinhard Simon  Thorsten Brinkhoff 《Ocean Dynamics》2009,59(2):291-304

Most-probable-number (MPN) dilution series were used to enumerate and isolate bacteria from bulk water, suspended aggregates, the oxic layer, and the oxic–anoxic transition zone of the sediment of a tidal flat ecosystem in the southern North Sea. The heterotrophic aerobic bacteria were able to grow on agar-agar, alginate, cellulose, chitin, dried and ground Fucus vesiculosus, Marine Broth 2216, palmitate, and starch. MPN counts of bulk water and aggregate samples ranged between 0.18?×?10¹ and 1.1?×?10⁶ cells per milliliter and those of the sediment surface and the transition zone between 0.8?×?10¹ and 5.1?×?10⁷ cells per gram dry weight. Marine Broth and F. vesiculosus yielded the highest values of all substrates tested and corresponded to 2.3–32% of 4,6-diamidinophenyl indole cell counts. Strains of seven phylogenetic classes were obtained: Actinobacteria, Bacilli, α- and γ-Proteobacteria, Sphingobacteria, Flavobacteria, and Planctomycetacia. Only with agar-agar as substrate could organisms of all seven classes be isolated.  相似文献   

Temporal changes in Sphingomonas and Mycobacterium populations in mangrove sediments contaminated with different concentrations of polycyclic aromatic hydrocarbons (PAHs)     

Guo C  Ke L  Dang Z  Tam NF 《Marine pollution bulletin》2011,62(1):133-139

The change in community diversity and structure of the indigenous, dominant, polycyclic aromatic hydrocarbon (PAH)-degrading bacterial genera, Sphingomonas and Mycobacterium, due to contamination in the environment is not very well known. A combination of PCR-DGGE with specific primers and a cultivation-dependent microbiological method was used to detect different populations of Sphingomonas and Mycobacterium in mangrove sediments. The structure of the entire bacterial community (including Sphingomonas) did not show a shift due to environmental contamination, whereas the diversity of Mycobacterium populations in mangrove sediments with higher PAH contamination increased from exposure between Day 0 and Day 30. The isolated Mycobacterium strains migrated to the same position as the major bands of the bacterial communities in Mycobacterium-specific DGGE. A dioxygenase gene system, nidA, which is commonly found in PAH-degrading Mycobacterium strains, was also detected in the more highly contaminated sediment slurries. The present study revealed that Mycobacterium species were the dominant PAH-degraders and played an important role in degrading PAHs in contaminated mangrove sediments.  相似文献   

新疆伊犁盆地511砂岩型铀矿床成矿作用机理研究   总被引：5，自引：1，他引：5  

王正其  李子颖  管太阳  张国玉 《矿床地质》2006,25(3):302-311

为探讨511铀矿床的形成机理,通过野外岩心观察和系统样品采集,结合室内化学分析、扫描电镜、电子探针等方法,详细研究了赋存于Ⅴ旋回砂体的铀矿地质特征,对砂岩铀的成矿作用展开了初步讨论。认为Ⅴ旋回层间氧化带可划分为氧化带、氧化_还原过渡带(包括褪色亚带和铀矿石亚带)和还原带;铀矿赋存在过渡带砂岩中,空间展布严格受层间氧化带控制;铀主要以显微浸染状铀矿物(沥青铀矿 铀石)形式散布于炭屑木质细胞腔内壁和砂岩碎屑颗粒的填隙物中,部分以吸附形式赋存于粘土矿物和炭屑;层间氧化带发育是含氧层间承压水与砂岩之间水岩作用的结果。伴随砂岩中有机质、S2-和Fe2 被氧化,形成的有机酸类、SO2-4和Fe3 等组分部分迁移至过渡带沉淀,也是砂岩中铀元素活化迁移、沉淀富集,再活化、再富集的循环过程;层间氧化带及其砂岩铀成矿作用发生于表生环境,始于喜马拉雅早期(66Ma),具有长期性和滚动式向前发展的特点;铀成矿可能与微生物细菌活动及其还原作用密切相关。  相似文献   

Geochemistry of porewater and sulfate-reducing bacteria in sediment cores from the Chianan Plain, Taiwan     

Lichun LIU Yuting CHEN Kuanyu CHEN Jyhyih LEU Tsungkwei LIU 《中国地球化学学报》2006,25(B08):67-67

High concentrations of arsenic and humic substances in groundwater from the southwestern coastal plain of Taiwan were well known for their probable relationships with black-foot disease. In order to realize the relationships between the concentrations of humic substances and arsenic in this area, 24 well water samples were analyzed. After filtered through 0.45 μm glass fiber membrane in the field, samples were kept in the dark at 4℃ and then separated into six fractions with varying range of molecular weight （〈0.5, 0.5-1, 1-5, 5-10, 10-50 and 〉50 k Da） by ultrafiltration apparatus （Molecular/Por Stirred Cell system） in the lab. Concentrations of humic substances were measured by fluorescence spectrometer （HITACHI F-2000, ex=370, em=445） and arsenic by FIAS-AA （Perkin Elmer AAnalyst 100, FIAS-400）. On average, only 6.2% of the total arsenic in water existed in the fraction of 〉0.5 k Da. and the others were complexed with humic substances （including humic acid and fulvic acid）. The results demonstrated a distinct positive correlation between the concentrations of humic substances and arsenic.  相似文献   

Characterization of microbial communities associated with African desert dust and their implications for human and ecosystem health     

Christina A. Kellogg 《中国地球化学学报》2006,25(B08):218-219

Desert winds aerosolize several billion tons of soil-derived dust each year, including concentrated seasonal pulses from Africa and Asia. Huge dust events create an atmospheric bridge over continents and oceans, and eject a large pulse of soil-associated microorganisms into the atmosphere. These dust events might therefore have a role in expanding the biogeographical range of some microorganisms by facilitating rare long-distance dispersal events. The goal of this study is to characterize the microbes associated with African dust events and determine if they pose a risk to humans or downwind ecosystems. Air samples were collected by vacuum filtration in a source region （Mali, West Africa） during dust events and plated on R2A media to culture microorganisms. These organisms were compared to those in similar samples collected in the Caribbean during Saharan/Sahelian dust events. A high-volume liquid impinger is currently being tested in Barbados, collecting aerosol samples during African dust events. Over 100 bacteria and fungi （19 genera of bacteria and three genera of fungi） have been characterized from source region dust events.  相似文献   

XANES analysis of the mechanisms of arsenic removal in biological iron and manganese treatment unit     

Yoko Fujikawa Daisuke Yoneda Atushi Minami Hiroshi Yashima Toshio Tonokai Sotoji Tani Masami Fukui Tatuhide Hamasaki Masataka Sugahara 《中国地球化学学报》2006,25(B08):113-113

Biological iron and manganese removal utilizing indigenous iron and manganese oxidizing bacteria （IRB hereafter） in groundwater can also be applied to arsenic removal according to our pilot-scale test. The arsenic removal probably occurred through sorption and complexation of arsenic to iron and manganese oxides formed by enzymic action of IRB. We investigated the chemical properties of iron and manganese oxides in IRB floc and the valence state of arsenic sorbed to the floc to clarify the mechanisms of the arsenic [especially As （Ⅲ）] removal. The floc samples were collected from two drinking water plants using IRB （Jyoyo and Yamatokoriyama, Japan）, and our pilot - scale test site where arsenic and iron removal using IRB is under way （Mukoh, Japan）. The Jyoyo and Yamatokoriyama IRB floc samples were subjected to As （Ⅲ） and As（Ⅴ） sorption experiments. The elemental composition of the floc samples was measured. XANES spectra were collected on As, Fe and Mn K-edges at synchrotron radiation facility Spring 8 （Hyogo, Japan）. FT-IR and the X-ray diffraction spectra of the samples were also obtained. The IRB floc contained ca. 35 % Fe, 0.3%-3.5% Mn and 2%-6% P. The samples were highly amorphous and contained ferrihidrites and hydrated iron phosphate. According to XANES analyses of IRB, As associated with IRB was in ＋5 valence state when As （Ⅲ） （or As （Ⅴ）） was added in laboratory sorption test, Fe in ＋3 valence state, and Mn a mixture of＋3 and ＋4 valence states. Small shift was observed in the XANES spectra of IRB on As K-edge as the equilibration time of the sorption experiment was increased. Gradual oxidation of a small amount of As （Ⅲ） associated with IRB or change in arsenic binding with sorption site were the probable mechanism.  相似文献   

Principal stages in evolution of Precambrian organic world: Communication 1. Archean and Early Proterozoic     

V. N. Sergeev  M. A. Semikhatov  M. A. Fedonkin  A. F. Veis  N. G. Vorob’eva 《Stratigraphy and Geological Correlation》2007,15(2):141-160

Expounded in this work are the results of critical consideration of published and original data on biologic nature and appearance chronology of different groups of Archean and Lower Proterozoic (3.5–1.65 Ga) paleontological remains known from geological record. Conclusions are substantiated by morphological analysis of structurally preserved microfossils, their facies distribution, and by inferable genesis and principal evolutionary trends of Archean stromatolites. A special attention is paid to variations of organic and carbonate carbon isotope composition in sedimentary successions with paleontological remains and to recent information about discovered, most ancient biomarkers of large groups of organic world. As a result of this approach, a detailed model of Precambrian organic world evolution is suggested.  相似文献   

南极菌Pseudoalteromonas sp．S-15-13产胞外多糖的研究及其分子鉴定   总被引：2，自引：1，他引：2       下载免费PDF全文

李江  陈靠山  林学政  李光友 《极地研究》2006,17(2):130-135

本文对影响南极细菌S-15-13生长和胞外多糖产量的主要环境因子进行了研究,同时采用16S rDNA序列分析及系统发育分析方法对其进行了分子鉴定。结果表明：菌株S-15-13最佳产糖条件为：培养时间,56h；培养温度,8℃；碳源,1．0%葡萄糖；NaCl浓度,3．0%；pH,6．0-7．0。16S rDNA序列分析和系统发育分析表明,菌株S-15-13属于假交替单胞菌属(Pseudoaltero- monas)。  相似文献